Services

Our Services

We offer the following services upon request:

  1. Providing inner ear celloidin sections mounted in glass slides.
  2. Step by step protocol for celloidin removal, antigen retrieval.
  3. Immunohistochemistry protocols to stain human inner ear tissue (light and fluorescent microscopy).
  4. Pilot testing for primary antibodies. Some antibodies required different type of antigen retrieal method, our laboratory can perform a pilot test to determine the method to follow for specific immunogen.
  5. Microdissected auditory and vestibular endorgans. The investigator may request auditory and vestibular endorgans microdissected for whole mount immunostaining.
  6. Cryostat sections from microdissected endorgans.
  7. Specific protocol for whole mount staining (cochlea or vestibular endorgans).
  8. Transmission electron microscopy processing for human inner ear, celloidin embedded tissue and microdissected endorgans. Protocols for plastic embedding are also available.
  9. Imaging protocols using high resolution laser confocal microscopy.
  10. Tissue sections available for histopathological analysis. Hematoxylin and Eosin collection of temporal bones with different inner ear disease, cochlea implanted and normal aging. Sections are loan for analysis.

Investigators can consult Dr. Lopez at laLopez@mednet.ucla.edu for specific protocols and experimental design before any staining is beginning.

Request Tissue

To request celloidin inner ear tissue sections for research purposes, please contact Ivan A. Lopez, Ph.D.

Phone: 310-825-5331
Email: ilopez@ucla.edu
Email: laLopez@mednet.ucla.edu

Training

We offer the following training:

  1. Celloidin embedding of human temporal bones.
  2. Celloidin sectioning.
  3. Hematoxylin and eosin staining.
  4. Human inner ear tissue handling.
  5. Human inner ear storing and archiving.

Consultations

For technical training on celloidin embedding and cutting, investigators can consult Ana Cordero at ACorderoSosa@mednet.ucla.edu.

For temporal bone donation please consult Ms. Marian Rush at MRush@mednet.ucla.edu.